TY - JOUR A1 - Sayed, Inas A1 - Saafan, Ali A1 - Abdel-Gawad, Fagr A1 - Harhash, Tarek A1 - Abdel-Rahman, Mostapha T1 - Effect of low-level laser therapy on gene expression of vascular endothelial growth factor and interleukin-1 ß in scalpel-induced and laser-induced oral wounds in rats Y1 - 2015/1/1 JF - Journal of Dental Lasers JO - J Dent Lasers SP - 23 EP - 30 VL - 9 IS - 1 UR - http://www.jdentlasers.org/article.asp?issn=2321-1385;year=2015;volume=9;issue=1;spage=23;epage=30;aulast=Sayed DO - 10.4103/0976-2868.157595 N2 - Introduction: The effect of low-level laser therapy (LLLT) on wound healing has been evaluated in several studies; however, little is known about the molecular mechanisms that underlies the biostimulatory effect of this treatment modality. Aim: to evaluate the effect of LLLT on gene expression of vascular endothelial growth factor (VEGF) and interleukin-1beta (IL-1 b) during healing of wounds created by surgical blade and by surgical laser in order to have a better image of their interactive role influenced by the laser irradiation. Materials and Methods: 40 male Wister rats were randomly assigned into four groups (A, B, C and D). In groups A and C, an incision was made in the gingival and mucosal tissues using a surgical scalpel. A similar incision was made in groups B and D using 980 nm diode laser. Group A and B were subjected twice to low level 870 nm diode laser 3 h and 24 h after the incision while group C and D served as controls. 30 min after the second irradiation, the rats belonging to all groups were euthanized, and the wound area was excised. Quantitative reverse-transcriptasepolymerase chain reaction was used to measure the gene expression of VEGF and IL-1b. Results and Discussion: LLLT caused an increase in VEGF gene expression in scalpel-induced wounds and a corresponding decrease in laser-induced wounds but without significant differences. LLLT inhibited the gene expression of IL-1b in both types of wounds, but this inhibition was only significant in scalpel wounds. Conclusion: Biostimulatory effect of LLLT could be mediated through modulation of the immune response by the inhibition of IL-1b. ER -